Mikrovalovima potpomognuta ekstrakcija bioaktivnih spojeva iz odabranih morskih algi i spužve

master's thesis

• Title (english): Microwave-assisted extraction of bioactive compounds from selected marine algae and sponge
• Author: Željka Špoljar
• Mentor: Sandra Pedisić (mentor)
• Mentor: Zrinka Čošić (pomoć pri izradi, neposredni voditelj)
• Committee member: Maja Repajić (predsjednik povjerenstva)
• Committee member: Ivona Elez Garofulić (član povjerenstva)
• Committee member: Verica Dragović-Uzelac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for drying Technologies and monitoring of biologically active compounds); Zagreb
• Defense date and country: 2023-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Cilj ovog rada bio je utvrditi maseni udio ukupnih fenola i pigmenata u ekstraktima morske spužve i raznih vrsta algi dobivenih ekstrakcijom uz pomoć mikrovalova. Više uzoraka algi vrsta Fucus virsoides i Cystoseira barbata uzorkovano je u različito vrijeme. Ukupni fenoli određeni su spektrofotometrijski u analiziranim uzorcima u rasponu od 34,6 do 3607,8 mg/100 g s.tv. HPLC metodom određeni su i ukupni karotenoidi (13,83-770,95 mg/100 g s.tv.) i klorofili (1,34-292,01 mg/100 g s.tv.). Antioksidacijska aktivnost određena je ORAC (119,33-411,72 µmol Trolox/100 g s.tv.), ABTS (96,40-6473,86 µmol Trolox/100 g s.tv.) i DPPH metodom (1733,38-3200,85 µmol Trolox/100 g s.tv.). Utvrđene su statistički značajne razlike u masenim udjelima ukupnih fenola, karotenoida i klorofila u ispitivanim uzorcima. Maseni udio ukupnih fenola u smeđim algama Fucus virsoides i Cystoseira barbata varirao je ovisno o godišnjem dobu i bio je najveći u jesen.

Abstract (english)

The aim of this study was to determine the mass fraction of total phenols and pigments in extracts of marine sponge and various algal species obtained by microwave-assisted extraction. Several samples of algae species Fucus virsoides and Cystoseira barbata were sampled at different times. Total phenols were determined spectrophotometrically in the analyzed samples and ranged from 34.6 to 3607.8 mg/100 g dm. Total carotenoids (13.83-770.95 mg/100 g dm) and chlorophylls (1.34-292.01 mg/100 g dm) were also determined by the HPLC method. Antioxidant activity was determined by ORAC (119.33- 411.72 µmol Trolox/100 g dm), ABTS (96.40-6473.86 µmol Trolox/100 g dm) and DPPH method (1733.38-3200.85 µmol Trolox/100 g dm). Statistically significant differences were found in the mass fractions of total phenolics, carotenoids and chlorophyll in the studied samples. The mass fraction of total phenolics in the brown algae Fucus virsoides and Cystoseira barbata varied depending on the season and was highest in autumn.

• Keywords: antioksidacijska aktivnost; ukupni fenoli; pigmenti; alge; spužva
• Keywords (english): antioxidant activity; total phenols; pigments; algae; sponge
• Language: croatian
• URN:NBN: urn:nbn:hr:159:196651
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-07-03 10:16:43