Utjecaj toplinske i ultrazvučne ekstrakcije na prinos proteina i fenola iz osušenih peleta lišća šećerne repe

master's thesis

• Title (english): Influence of thermal and ultrasonic extraction on protein and phenolycs yield from dried sugar beet leaf pellets
• Author: Sven Kovačićek
• Mentor: Anet Režek Jambrak (mentor)
• Mentor: Josipa Dukić (pomoć pri izradi, neposredni voditelj)
• Committee member: Sven Karlović (predsjednik povjerenstva)
• Committee member: Mojca Čakić Semenčić (član povjerenstva)
• Committee member: Jasenka Gajdoš Kljusurić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Management, Foreign Languages for Specific Purposes and Physical Education and Health); (Laboratory for Sustainable Development); Zagreb
• Defense date and country: 2024-11-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

List šećerne repe je zbog svojeg povoljnog sastava proteina i fenolnih spojeva prepoznat kao vrijedan otpad industrije šećera s potencijalom valorizacije. Cilj rada je usporediti utjecaj toplinske i ultrazvučne metode ekstrakcije uz primjenu zelenih otapala na prinos ukupnih proteina i fenola te njihovu antioksidacijsku aktivnost iz osušenih peleta lišća šećerne repe. Primjena ultrazvučne ekstrakcije rezultirala je najvećim prinosom ukupnih proteina (55,38 ± 2,57 mg BSA/g s.tv.) i fenola (6,23 mg ± 0,56 mg GAE/g s.tv.). Pri ultrazvučnoj ekstrakciji utvrđen je statistički značajan (p<0,05) utjecaj amplitude, vremena tretiranja i udjela etanola na prinos ukupnih proteina, odnosno statistički značajan (p<0,05) utjecaj amplitude i trajanja tretmana na prinos ukupnih fenola. Primjena maksimalne amplitude (100 %) tijekom 9 minuta i deionizirane vode kao otapala pokazala se optimalnom kombinacijom za ultrazvukom potpomognutu ekstrakciju proteina i fenola iz osušenih peleta. Ultrazvuk visokog intenziteta pokazao se kao efikasna netoplinska metoda ekstrakcije iz osušenih peleta lišća šećerne repe.

Abstract (english)

Due to favorable composition of proteins and phenolic compounds, the sugar beet leaf is a valuable by-product of the sugar industry. Thesis aims to compare the influence of thermal and ultrasonic extraction methods using green solvents on the yield of total proteins and phenols, and their antioxidant activity, from dried pellets of sugar beet leaves. Ultrasonic extraction resulted in the highest yield of total proteins (55,38 ± 2,57 mg BSA/g d.m.) and phenols (6,23 mg ± 0,56 mg GAE/g d.m.). During ultrasonic extraction, a statistically significant (p<0,05) influence of the amplitude, treatment time and the proportion of ethanol on the yield of total proteins was observed, i.e. a statistically significant (p<0,05) influence of amplitude and treatment time was observed on the yield of total phenols. Application of maximum amplitude (100 %) for 9 minutes and deionized water as a solvent proved optimal for ultrasound-assisted extraction of proteins and phenols from dried pellets.

• Keywords: ultrazvukom potpomognuta ekstrakcija; list šećerne repe; proteini; fenoli; otapalo
• Keywords (english): ultrasound assisted extraction; sugar beet leaf; proteins; phenols; solvent
• Language: croatian
• URN:NBN: urn:nbn:hr:159:705974
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-11-25 09:29:29