Proizvodnja arabitola iz glicerola pomoću kvasca Debaryomyces hansenii JCM 1990

master's thesis

• Title (english): Production of arabitol from glycerol by yeast Debaryomyces hansenii JCM 1990
• Author: Toni Vlahović
• Mentor: Mario Novak (mentor)
• Mentor: Nenad Marđetko (pomoć pri izradi, neposredni voditelj)
• Committee member: Antonija Trontel (predsjednik povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Committee member: Blaženka Kos (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2024-11-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Zbog modernizacije, ljudi su uvijek u potrazi za novim obnovljivim izvorima energije. Glicerol, nusproizvod industrije biodizela, predstavlja zanimljivu priliku za pretvaranje proizvoda niske vrijednosti u proizvode kao što su arabitol i ksilitol, visokovrijedni šećerni alkoholi koji se koriste u raznim industrijama, uglavnom u prehrambenoj industriji kao umjetni zaslađivači. Kemijska sinteza navedenih tvari često je skupa, pa se razmatra biotehnološki pristup. Debaryomyces hansenii, ne-Saccharomyces kvasac, ima sposobnost sintetizirati arabitol koristeći glicerol kao supstrat. Svrha ovog rada bila je odrediti optimalnu pH vrijednost, početnu koncentraciju glicerola i koncentraciju natrijevog klorida za proizvodnju arabitola pomoću kvasca Debaryomyces hansenii JCM 1990. Najveća produktivnost (Prarabitol = 0,035 g/Lh) kao i najveći prinos (Yarabitol = 4,235 g/L) uočeni su u mediju s 50 g/L glicerola, 0 g/L NaCl i pH vrijednosti 6.

Abstract (english)

Abstract: Due to modernization, humans are always searching for new renewable energy sources. Glycerol, a byproduct of the biodiesel industry, presents an interesting opportunity to turn low value product into products such as arabitol and xylitol, which are high value sugar alcohols that are used in various industries, mainly in food industry as artificial sweeteners. Chemical synthesis of said substances is often expensive, so a biotechnological approach is considered. Debaryomyces hansenii, a non-Saccharomyces yeast, has a capability to synthesize arabitol using glycerol as substrate. The purpose of this paper was to determine the optimal pH level, initial glycerol concentration and sodium chloride concentration for arabitol production using the yeast Debaryomyces hansenii JCM 1990. Highest productivity (Prarabitol = 0,0353 g/Lh) as well as highest yield (Yarabitol = 4,235 g/L) were observed in medium with 50 g/L glycerol, 0 g/L NaCl and a pH level of 6.

• Keywords: glicerol; arabitol; šećerni alkoholi; Debaryomyces hansenii; bioreaktor s miješalom
• Keywords (english): glycerol; arabitol; sugar alcohols; Debaryomyces hansenii; stirred-tank bioreactor
• Language: croatian
• URN:NBN: urn:nbn:hr:159:302590
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-12-03 14:29:25