Bioaktivni potencijal maka (Papaver somniferum )

master's thesis

• Title (english): Bioactive potential of poppy extract (Papaver somniferum )
• Author: Ivana Čigir
• Mentor: Ksenija Durgo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2015-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U današnje vrijeme mnogo se istraživanja provodi na biljnim vrstama koje sadrže različite biološki aktivne tvari, no do sada nisu sve dovoljno istražene. Mak je jedna od navedenih biljnih vrsta, koja zbog svojeg sastava ima mnoge potencijalno povoljne učinke na ljudski organizam. S ciljem dobivanja boljeg uvida u djelovanje ekstrakta deproteiniziranog maka na humane kancerogene stanice HEp2 i A549, testirana je citotoksičnost, indukcija slobodnih radikala, učinak na lipidnu peroksidaciju te je određivan ukupan udjel polifenolnih spojeva. Rezultati pokazuju da je u višim koncentracijama uzorak deproteiniziranog maka citotoksičan. Pri nižim koncentracijama na obje stanične linije, uzorak ima antioksidativno djelovanje, dok pri višim koncentracijama djeluje prooksidativno. Rezultati su pokazali kako se uklanjanjem proteina iz uzorka, uklanjaju i polifenolni spojevi.

Abstract (english)

There is a lot of research focusing on plants consisting of various biologicaly active compounds but their activity still needs to be proven. Poppy is known for it's benefitial effects on human organism. In order to get a better insight of deoproteinized poppy’s activity on human cancer cells HEp2 and A549, cytotoxicity has been tested, free radicals induction, effect on a lipid peroxidation and total polyphenols content was determined. The results has shown that high concentration of deoproteinized poppy is cytotoxic. Lower concentration on both of the cell lines has shown antioxidative effect, while high concentration acts pro oxidative. Results showed that removal of proteins from pattern can cause removal and polyphenolic compounds.

• Keywords: mak (Papaver somniferum); stanice HEp2; stanice A549; citotoksičnost; lipidna peroksidacija
• Keywords (english): poppy (Papaver somniferum); HEp2; A549; cytotoxicity; lipid peroxidation
• Language: english
• URN:NBN: urn:nbn:hr:159:146764
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 09:24:11