Biološki učinak čajeva na stanične linije probavnog sustava

master's thesis

• Title (english): Biological effects of tea on cell lines of the digestive system
• Author: Tanja Pijerov
• Mentor: Ksenija Durgo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2013-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Čaj se dobiva iz biljke Camellia sinensis i bogat je flavonoidima, spojevima koji pokazuju širok spektar bioloških i farmakoloških učinaka u organizmu. Cilj ovog rada bio je ispitati citotoksično, prooksidativno i antioksidativno djelovanje pet vrsta čaja (bijeli, žuti, zeleni, oolong i crni) na dvije stanične linije: ljudske stanice karcinoma grkljana (HEp2) i ljudske stanice karcinoma debelog crijeva (CaCo2). Ispitan je i sadržaj proteina u CaCo2 stanicama tretiranim s žutim čajem i uspoređen sa sadržajem proteina u netretiranim stanicama. Rezultati su pokazali da se povećanjem koncentracije čajeva povećava citotoksičnost i da je stanična linija CaCo2 rezistentnija na citotoksično djelovanje čajeva u odnosu na HEp2 staničnu liniju. Također je pokazano da čajevi djeluju prooksidativno pri koncentracijama nižim od 1x i da je jače prooksidativno djelovanje detektirano na CaCo2 staničnoj liniji. Tretiranje CaCo2 stanica sa žutim čajem uzrokovalo je sintezu proteina uključenih u obranu od staničnog stresa i reguliranje apoptoze. Kod koncentracije 0,1x sintetiziran je TCP1, protein uključen u smatanje drugih proteina koji se pojačano sintetizira prilikom staničnog stresa. Kod koncentracije 0,125 x sintetizirana su dva proteina: neimenovani proteinski produkt i hnRNPA2/B1, uključen u regulaciju staničnog ciklusa. Kod koncentracije 0,25x sintetizirano je pet proteina: hnRNPA3 koji održava stabilnost telomera; S100A4, uključen u regulaciju apoptoze i staničnog preživljenja; GAPDH, enzim glikolize koji može inducirati apoptozu; MTHSP75, uključen u smatanje i razmatanje drugih proteina te Ciklofilin A u kompleksu sa dipeptidom glicil-prolin (Gly-Pro), izomeraza koja katalizira izomerizaciju peptidnih veza i olakšava smatanje proteina.

Abstract (english)

Tea is produced from the plant Camellia sinensis and contains flavonoids, compounds which show a wide specter of biological and pharmacological effects in the organism. The aim of this study was to evaluate cytotoxic, antioxidant and prooxidant effects of five types of tea (white, yellow, green, oolong and black) on two human cell lines: laryngeal carcinoma cell line (HEp2) and colorectal adenocarcinoma cell line (CaCo2). Protein content of CaCo2 cells treated with yellow tea was also evaluated and compared to the protein content of untreated cells. The results showed that increasing the tea concentratios led to increased cytotoxicity and also that CaCo2 cell line was more resistant to the cytotoxic effect of tea compared to HEp2 cell line. It was also shown that tea concentrations lower than 1x have prooxidative effects and that stronger prooxidative effects were detected on CaCo2 cell line. Treatment of CaCo2 cells with yellow tea caused overexpression of proteins involved in defence against cell stres and regulation of apoptosis. Tea concentration 0,1x led to overexpression of TCP1, a protein involved in folding other proteins, which is overexpressed during cell stress. Tea concentration 0,125x led to overexpression of two proteins: an unnamed protein product and hnRNPA2/B1, which is involved in regulation of cell cycle. Tea concentration of 0,25 x led to overexpression of five different proteins: hnRNPA3, which maintains telomere stability; S100A4, involved in regulation of apoptosis and cell survival; GAPDH, a glycolysis enzyme which can induce apoptosis; MTHSP75, involved in folding and unfolding of other proteins and Cyclophillin A complexed with dipeptide Gly-Pro, an isomerase enzyme, which catalizes the isomerization of peptide bonds and facilitates protein folding.

• Keywords: čaj; flavonoidi; citotoksičnost; (anti)oksidativno djelovanje; proteini
• Keywords (english): tea; flavonoids; cytotoxicity; (anti)oxidative effects; proteins
• Language: croatian
• URN:NBN: urn:nbn:hr:159:458010
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-05 11:12:36