Utjecaj suplementarnih vazodilatacijskih stimulansa na oksidacijski antioksidacijski status u Swiss albino miša

master's thesis

• Title (english): Supplementary vasodilation stimulants effect on oxidative/antioxidative status in Swiss albino mice
• Author: Marta Vuković
• Mentor: Irena Landeka Jurčević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Chemistry and Biochemistry); Zagreb
• Defense date and country: 2013-07-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Erektilna disfunkcija (ED) postaje sve važniji zdravstveni problem, koji smanjuje kvalitetu života. Danas se smatra da ED nije bolest nego jedan od najranijih simptoma endotelne disfunkcije i bolesti krvnih žila te jedan od najvažnijih prediktora razvoja teže kardiovaskularne bolesti. Terapija prvog izbora u liječenju ED-a, neovisno o etiologiji, jesu inhibitori fosfodiesteraze 5. Sve je veći interes i u Hrvatskoj za liječenje prirodnim metodama uključujući i biljnih dodataka prehrani. Ovaj rad istražuje utjecaj suplementarnih vazodilatacijskih stimulansa na oksidacijskiantioksidacijski status u jetri, bubrezima i testisima Swiss albino miša mjerenjem aktivnosti antioksidacijskih enzima reduciranog glutationa (GSH) i superoksid dismutaze (SOD). Peroksidacija lipida također je procijenjena mjerenjem koncentracije MDA, kao biomarkera oksidacijskog stresa. Rezultati pokazuju da značajno povećava koncentraciju MDA u jetri, bubrezima i testisima za dva testirana dodatka prehrani. Isto tako, aktivnosti enzima SOD i GSH za sva tkiva su statistički značajno smanjeni (p <0,05) u usporedbi s kontrolnom skupinom.

Abstract (english)

Although erectile dysfunction (ED) is a benign disorder, it affects physical and psychosocial health and has a significant impact on the quality of life. Today, ED is not considered a disease but one of the very early symptoms of cardiovascular disease, and it is in most cases caused by organic changes. Phosphodiesterase-5 inhibitors are considered to be the first line treatment for all types of ED, regardless of its cause. Growing number of people in Croatia are using varieties of alternative and complementary forms of natural therapies including herbal medicines. The present study investigates the supplementary vasodilatation stimulants effect on oxidative/antioxidative defence systems of liver, kidney and testes tissues of Swiss albino mice by measuring the reduced glutathione (GSH) and superoxide dismutase (SOD) activity. Lipid peroxidation was also estimated by measuring the MDA levels, as biomarker of oxidative stress. The results revealed that significantly increased MDA levels in liver, kidney and testes for the two tested supplements. Similarly, SOD and GSH activities for all tissues reduced significantly (p<0.05) compared to their corresponding control.

• Keywords: dodaci prehrani; lipidna peroksidacija; superoksid dismutaza; reducirani glutation
• Keywords (english): supplements; lipid peroxidation; superoxide dismutase; reduced glutathione
• Language: croatian
• URN:NBN: urn:nbn:hr:159:452455
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-05 12:15:53