Istraživanje genotoksičnog, antioksidativnog i prooksidativnog učinka kvercetina na ljudskim stanicama karcinoma grkljana (HEp2)

master's thesis

• Title (english): Research of genotoxic, antioxidative and pooxidative effects of quercetin on human laryngeal carcinoma (HEp2) cells
• Author: Maja Ivelić
• Mentor: Ksenija Durgo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2012-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Kvercetin, flavonoid koji je u visokoj koncentraciji prisutan u luku, jabukama, grožđu i drugim namirnicama, karakterizira nekoliko bioloških učinaka: antioksidativno djelovanje, protuupalna, antivirusna i antitumorska aktivnost. Cilj ovoga rada je bio ispitati citotoksično djelovanje kvercetina na humanim stanicama karcinoma grkljana (HEp2) i utvrditi koju vrstu stanične smrti izaziva ovaj spoj u širokom rasponu koncentracija (1-3000 µM). Također, na temelju jednolančanih i dvolančanih lomova izazvanih ovim spojem utvrđen je mutageni, prooksidativni i antioksidativni potencijal kvercetina. Citotoksičnost kvercetina je ovisna o koncentraciji i vremenu inkubacije HEp2 stanica, a značajno smanjenje preživljenja stanica zapaženo je kod koncentracije 100 µM i viših koncentracija. Nadalje, izlaganje stanica kvercetinu uzrokuje apoptozu i nekrozu, s tim da je apoptoza bila dominantan način stanične smrti. Oštećenja DNA izazvana kvercetinom ovisila su o koncentraciji i vremenu inkubacije. Rezultati pokazuju da ovaj spoj inhibira oštećenje DNA izazvano vodikovim peroksidom u koncentraciji 20 µM i da u toj koncentraciji sam ne izaziva oštećenja DNA.

Abstract (english)

Quercetin, a flavonoid present in high concentrations in onion, apples, grapes and other food products, is characterized by several biological effects: antioxidant activity, antiinflammatory, antiviral and anticancer activity. The aim of this study was to investigate the cytotoxic effect of quercetin on human laryngeal carcinoma cells (HEp2) and to determine the type of cell death caused by this compound in a wide concentration range (1-3000 µM). Also, its ability to induce/prevent single and double strand DNA breaks was determined. In order to determine its antioxidative nature, inhibition of DNA breaks was determined in the cells previously treated with H2O2. The cytotoxicity of quercetin is dependent on the concentration and time of incubation HEp2 cells, a significant decrease in cell survival was observed at concentrations of 100 µM and higher. The dominant mode of cell death caused with quercetin is apoptosis. It causes DNA breaks time- and concentration-dependent manner. Quercetin inhibits hydrogen peroxide-induced DNA damage at concentration of 20 µM and does not cause DNA damage itself.

• Keywords: kvercetin; apoptoza; nekroza; NR test; komet test
• Keywords (english): quercetin; apoptosis; necrosis; NR assay; comet assay
• Language: croatian
• URN:NBN: urn:nbn:hr:159:331325
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-08 10:23:51