Synthesis and functionalization of porous silicon nanoparticles for nucleic acid delivery and photodynamic therapy

diplomski rad

• Autor: Petra Ivašković
• Mentor: Vladimir Mrša (mentor)
• Ustanova koja je dodijelila akademski / stručni stupanj: Sveučilište u Zagrebu; Prehrambeno-biotehnološki fakultet; (Zavod za kemiju i biokemiju); (Laboratorij za biokemiju); Zagreb
• Datum i država obrane: 2013-07-01, Hrvatska
• Znanstveno / umjetničko područje, polje i grana: BIOTEHNIČKE ZNANOSTI; Biotehnologija

Sažetak

In recent years there has been a significant increase in research on biomedical applications of nanoparticles. They have attracted much attention due to their ability to encapsulate and release a diversity of therapeutics or genes to the organism in an effective and selective way. Among different nanocarriers, the porous silicon nanoparticles show some advantages due to their desirable properties such as biocompatibility, non-toxic degradation products, tunable pore sizes and volumes with a high payload capacity, a high surface area and an efficient photoluminescence. The aim of this project was functionalization of the porous silicon nanoparticles applied in two different cancer therapy approaches: immunotherapy and photodynamic therapy (PDT). The porous silicon nanoparticles were functionalized with histidine and lysine, the amino acids able to complex the nucleic acids. They were also functionalized with a photosensitizer porphyrin and the targeting agent, mannose. The nanoparticles were characterized by IR spectroscopy and quantification was done by UV-Vis spectroscopy. The effectiveness of functionalized nanoparticles was tested in vitro in two-photon excitation photodynamic therapy on MCF-7 cells.

• Ključne riječi: nanoparticles of porous silicon; surface modification; immunotherapy; photodynamic therapy; cancer
• Jezik: engleski
• URN:NBN: urn:nbn:hr:159:355437
• Studijski program: Naziv: Molekularna biotehnologija; Vrsta studija: sveučilišni; Stupanj studija: diplomski; Akademski / stručni naziv: magistar/magistra inženjer/inženjerka molekularne biotehnologije (mag. ing. biotechn.)
• Vrsta resursa: Tekst
• Način izrade datoteke: Izvorno digitalna
• Prava pristupa: Pristup korisnicima matične ustanove
• Datum i vrijeme pohrane: 2017-05-04 10:16:41