Priprava ekstrakata komine grožđa primjenom eutektičnih otapala i ispitivanje njihove biološke aktivnosti

master's thesis

• Title (english): Preparation of grape pomace extracts using eutectic solvents and testing their biological activity
• Author: Ana Piškulić
• Mentor: Kristina Radošević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2016-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu su pripremljeni ekstrakti komine grožđa s osam različitih eutektičnih otapala i s etanolom, zakiseljenim etanolom i vodom te su analizirani njihovi sastavi s obzirom na ukupne polifenole, antocijane te je određen antioksidacijski kapacitet. Biološka aktivnost pripremljenih ekstrakta komine grožđa ispitana je in vitro na trima staničnim linijama (MCF-7, HeLa, HEK293T). Ekstrakti komine grožđa u DES-ovima imaju slabiji citotoksični učinak na normalnu humanu staničnu liniju HEK293T, nego na tumorske stanice, što upućuje na njihov mogući antitumorski učinak. Ekstrakti komine grožđa u kolin klorid:jabučna kiselina i kolin klorid:prolin:jabučna kiselina imaju najviše vrijednosti antioksidativnog kapaciteta i masenih udjela polifenolnih spojeva i antocijana što ukazuje na to da su najbolji kandidati za daljni razvoj i primjenu u zelenoj ekstrakciji.

Abstract (english)

In this paper grape, pomace extracts are prepared by eight different deep eutectic solvents and ethanol, acidified ethanol and water and their composition is analysed considering the total polyphenols, anthocyanins and their antioxidant capacity is determined. The biological activity of the prepared extract of grape pomace is examined in vitro on three cell lines (MCF-7, HeLa, HEK293T). Extracts of grape pomace in DESs have weaker cytotoxic effect on normal human cell HEK293T, than on the tumor cells, which suggests their possible antitumor effect. Extracts of grape pomace in choline chloride: malic acid and choline chloride: proline: malic acid has the highest value of antioxidant capacity and the mass fraction of polyphenols and anthocyanins which indicate that they are the best candidates for further development and use in the green extraction.

• Keywords: antioksidacijski kapacitet; antocijani; eutektična otapala; komina grožđa; polifenolni spojevi
• Keywords (english): antioxidative capacity; anthocyanins; deep eutectic solvents; grape pomace; polyphenolic compounds
• Language: croatian
• URN:NBN: urn:nbn:hr:159:677071
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 10:34:15