Primjena hladne plazme u inaktivaciji mikroorganizma čiste kulture E.coli

undergraduate thesis

• Title (english): Application of cold plasma treatment on inactivation of microorganism E.coli in pure culture
• Author: Ana Mandura
• Mentor: Zoran Herceg (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Food Processes Engineering); Zagreb
• Defense date and country: 2016-07-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Cilj ovog rada bio je istražiti utjecaj hladne plazme na stupanj redukcije, oksidacijski stres stanica E. coli i fiziološke čimbenike (pH, temperaturu, konduktivnost i slobodnih radikale) tretiranih uzoraka. Uzorci su tretirani hladnom plazmom pri frekvencijama od 60, 90 i 120 Hz u trajanju od 5 i 10 min. Tehnologija hladne plazme pokazala se efikasnom za postizanje potpune redukcije broja mikroorganizama. Dužim tretmanom i povećanjem frekvencije, došlo je do smanjenja pH vrijednosti i povećanja temperature i konduktivnosti otopine. Također, utvrđen je povišeni oksidacijski stres u bakterijskim stanicama. Osim redukcije, nakon tretmana postignuto je istjecanje staničnih makromolekula kao posljedica elektroporacije stanica. Bakterijske stanice su se rekuperirale nakon 18 h pri temperaturi inkubacije od 30˚C.

Abstract (english)

The aim of this study was to investigate the influence of cold plasma treatmenton degree of reduction, oxidative stress of cells E. coli and physiological factors (pH, temperature, conductivity, free radicals ) in treated samples. The samples were treated with cold plasma at frequencies of 60, 90 and 120 Hz during 5 and 10 minutes. Cold plasma technology was proved as the effective method for complete reduction of microoganisms. Longer treatment time with higher frequency showed decrease of ph value, increase of temperature and solution conductivity. Also, increased oxsidative stress was found in bacterial cells. Except reduction, leakage of celluar macromolecules was achieved after treatment as a result of electroporation of cells.Cells were recovered after 18 h of incubation at 30 ˚C.

• Keywords: hladna plazma; E. coli; redukcija; oksidacijski stres; rekuperacija
• Keywords (english): cold plasma; E.coli; reduction; oxsidative stress; recuperation
• Language: croatian
• URN:NBN: urn:nbn:hr:159:740361
• Study programme: Title: Food Technology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 13:09:14