Izolacija i karakterizacija fenolnih spojeva iz ploda trnine

undergraduate thesis

• Title (english): Isolation and characterization of phenolic compounds from blackthorn fruit
• Author: Tamara Vulić
• Mentor: Jasmina Lapić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Organic Chemistry); Zagreb
• Defense date and country: 2017-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Trnina (Prunus spinosa L.) pripada rodu Prunus, podporodici Prunoideae, porodici Rosaceae. Plod trnine sadrži značajan udio bioaktivnih spojeva među kojima značajno mjesto zauzimaju fenoli. Stoga je cilj ovog završnog rada bio iz uzoraka ploda trnine izolirati bioaktivne komponente klasičnom ekstrakcijom grijanjem u povrat te ekstrakcijom po Soxhletu uz 50%-tni etanol otapalo. Dominantne komponente karakterizirane su pomoću FTIR–spektroskopije, pri čemu je dokazana prisutnost fenolnih spojeva. Određena masena koncentracija ukupnih fenola u ekstraktu dobivenom klasičnom ekstrakcijom iznosi 29,16 mg GAE/100 g trnine, a za ekstrakt dobiven ekstrakcijom po Soxhletu 7,12 mg GAE/100 g trnine.

Abstract (english)

Blackthorn (Prunus spinosa L.) is a species of Prunus, genus of Prunoideae, family of Rosaceae. Fruit of blackthorn contain a significant proportion of bioactive compounds,among them significant place have phenols. The purpose of this study was isolation of bioactivecomponents from fruit samples of blackthorn classical extractionand extracted using extraction by Soxhlet with 50% ethanol.Main components were identified FTIR-spectroscopy and thepresence of the phenolic compounds was shown. Certain mass concentration of total phenolics in the extracts obtained by classical extraction is 29.16 mg GAE / 100 g of blackthorn, and the extract obtained by extraction by Soxhlet 7.12 mg GAE / 100 g of blackthorn.

• Keywords: trnina; bioaktivne komponente; ekstrakcija; ukupni fenoli
• Keywords (english): blackthorn; bioactive components; isolation; total phenolics
• Language: croatian
• URN:NBN: urn:nbn:hr:159:295896
• Study programme: Title: Nutrition; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-09-19 12:12:15