Inhibicijski učinak fenolnih spojeva na rast plijesni Mortierella isabellina i sintezu lipida

undergraduate thesis

• Title (english): Inhibitory effect of phenolic compounds on growth of fungus Mortierella isabellina and lipid synthesis
• Author: Marija Kuzmić
• Mentor: Mirela Ivančić Šantek (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2017-09-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Lignocelulozne sirovine koriste se za proizvodnju biogoriva i biokemikalija. Zbog njihove složene strukture, prije samog bioprocesa, potrebno ih je podvrgnuti metodama predobrade i hidrolize. Tim postupcima nastaju, osim fermentabilnih šećera, brojni nusproizvodi od kojih neki mogu inhibirati radni mikroorganizam. Oleaginozna plijesan Mortierella isabellina ima sposobnost nakupljanja velikih količina intracelularnih lipida (do 80% suhe tvari biomase) što ju čini obećavajućim organizmom u proizvodnji biodizela. Cilj ovog rada bio je istražiti učinak fenolnih spojeva (vanilin i 4-hidroksibenzaldehid) nastalih tijekom predobrade lignocelulozne sirovine na rast M. isabellina i sintezu lipida. Rezultati su pokazali da vanilin i 4-hidroksibenzaldehid značajno inhibiraju rast plijesni i sintezu lipida. Pri koncentracijama vanilina i 4-hidroksibenzaldehida većim od 1,2 i 0,6 g L-1, rast i akumulacija lipida bili su u potpunosti inhibirani.

Abstract (english)

Lignocellulosic raw materials are used in production of biofuels and biochemicals. Because of their complex structure, prior to the bioprocess itself, they have to be subjected to pre-treatment and hydrolysis. These processes generate, along with fermentable sugars, numerous byproducts, some of which may inhibit the working microorganism in the production process. The oleaginous fungus Mortierella isabellina has the ability to accumulate large quantities of intracellular lipids (up to 80% of dry cell weight) that makes it a promising organism for use in biodiesel production. The aim of this paper was to investigate effect of phenolic compounds (vanillin and 4-hydroxybenzaldehyde) produced during lignocellulose pretreatment on the growth of M. isabellina and lipid synthesis. The results showed that vanillin and 4-hydroxybenzaldehyde significantly inhibit the fungus growth and lipid synthesis. At concentrations of vanillin and 4-hydroxybenzaldehyde higher than 1,2 and 0,6 g L-1 respectively, the growth and lipid accumulation was completely inhibited.

• Keywords: 4-hidroksibenzaldehid; lignoceluloza; Mortierella isabellina; predobrada; vanilin
• Keywords (english): 4-hydroxybenzaldehyde; lignocellulose; Mortierella isabellina; pretreatment; vanillin
• Language: croatian
• URN:NBN: urn:nbn:hr:159:170443
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-09-20 07:41:43