Spektrofotometrijsko određivanje polifenola u acetonskim ekstraktima ružmarina (Rosmarinus officinalis)

undergraduate thesis

• Title (english): Spectrofotometric determination of polyphenols from acetone extracts of rosemary (Rosmarinus officinalis)
• Author: Andrea Buratović
• Mentor: Antonela Ninčević Grassino (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Analytical Chemistry); Zagreb
• Defense date and country: 2017-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Budući da je vrlo mali broj znanstvenih radova pokazao da se biljni ekstrakti mogu koristiti kao potencijalni inhibitori korozije metala i metalnih legura, cilj ovog rada bio je pripremiti acetonske ekstrakte lišća ružmarina, koji će se koristiti u daljnjim postupcima antikorozijskog testiranja. Dakle, u ovom radu su pripravljeni acetonski (30 i 70 % v/v) ekstrakti lišća ružmarina primjenom ultrazvučne ekstrakcije pri vremenu od 3, 6 i 9 min i amplitudi od 60, 80 i 100 %. Također, upotrijebljena je i ekstrakcija refluksiranjem pri vremenu od 1,5 i 3 h. Maseni udio ukupnih fenola i flavonoida u pripravljenim ekstraktima određen je UV/Vis spektrofotometrijom. Na temelju dobivenih rezultata određeni su optimalni uvjeti ultrazvučne ekstrakcije (70 %-tni aceton, 100 %-tna amplituda i vrijeme od 9 min), koji će se koristiti u daljnjim pripravama ekstrakata pri ispitivanju njihova antikorozijskog djelovanja.

Abstract (english)

Due to the fact that few scientific papers have shown that plant extracts could be used as potential corrosion inhibitor, the object of this work was preparation of acetone rosemary extracts, which will be used in further process of anticorrosion testing. Therefore, in this work was prepared acetone (30 and 70 %, v/v) extracts of rosemary by ultrasound extraction, at time of 3,6 and 9 min and amplitude of 60, 80 and 100 %. The extraction by refluxing, at time of 1,5 and 3 h was applied, too. Mass fraction of total phenols and flavonoids in prepared extracts was determined by UV/Vis spectrophotometry. On the basis of obtained results, the optimal conditions of ultrasound extraction (70 % acetone, 100 % amplitude and time of 9 min) was determined, and will be used in further preparation of extracts for examination of theirs anticorrosion action.

• Keywords: ekstrakcija; korozija; polifenoli; ružmarin; ultrazvuk
• Keywords (english): corrosion; extraction; polyphenols; rosemary; ultrasound
• Language: croatian
• URN:NBN: urn:nbn:hr:159:344515
• Study programme: Title: Food Technology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-09-28 12:05:16