Utjecaj hladne plazme na inaktivaciju i oštećenje stanice kvasca Saccharomyces cerevisiae S288C

undergraduate thesis

• Title (english): The effect of cold plasma treatment on inactivation and damage of yeast Saccharomyces cerevisiae S288C
• Author: Ema Vrančić
• Mentor: Anet Režek Jambrak (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Food Processes Engineering); Zagreb
• Defense date and country: 2018-09-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Svrha ovog rada je odrediti utjecaj hladne plazme na stanice kvasca Saccharomyces cerevisiae S288C. Uzorci kvasca su tretirani različitim protocima plina argona (1, 1.5 i 2 L/min) uz različito vremensko trajanje tretmana (1, 3, 5 i 20 min). Uzorcima je prije i nakon tretmana mjerena temperatura, pH, provodljivost, apsorbancija pri 600 nm za određivanje broja stanica te apsorbancija pri 260 i 280 nm za određivanje istjecanja staničnog sadržaja. Rezultati su analizirani metodom odzivne površine (RSM) korištenjem programa STATGRAPHICS Centurion. Iz rezultata je vidljivo da nije došlo do inaktivacije stanica kvasca jer nije uočena razlika u broju živih stanica prije i nakon tretmana. Najveće stanično istjecanje DNA postignuto je u uzorku tretiranom 5 min protokom plina od 2 L/min gdje je došlo do porasta apsorbancije za 0.591, a najveće stanično istjecanje proteina postignuto je u uzorku tretiranom 1 min protokom plina od 1 L/min gdje je zabilježen porast apsorbancije za 0.635.

Abstract (english)

The aim of this study is to determine the effect of cold plasma on Saccharomyces cerevisiae S288C. Yeast samples were treated with different argon gas flow rates (1, 1.5 and 2 L/ min) with varying duration of treatment (1, 3, 5 and 20 minutes). Before and after treatment, samples were measured for pH, conductivity, absorbance at 600 nm for cell count and absorbance at 260 and 280 nm for determination of cellular leakage. The results were analyzed by the reaction surface method (RSM) using STATGRAPHICS Centurion program. The results showed that there was no cell inactivation because no difference in the number of live cells before and after treatment was shown. The highest cellular DNA leakage was achieved in a sample treated 5 minutes with a gas flow rate of 2 L/min where absorbance was increased by 0.591. The highest cellular protein leakage was achieved in a sample treated 1 minute with a gas flow rate of 1 L/min where absorbance was increased by 0.635.

• Keywords: hladna plazma; inaktivacija; istjecanje sadržaja; Saccharomyces cerevisiae
• Keywords (english): cell leakage; cold plasma; inactivation; Saccharomyces cerevisiae
• Language: croatian
• URN:NBN: urn:nbn:hr:159:043568
• Study programme: Title: Food Technology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-09-24 09:59:24