Izolacija i identifikacija antocijana iz komine grožđa primjenom eutektičnih otapala

master's thesis

• Title (english): Isolation and identification of anthocyanins from grape pomace using deep eutectic solvents
• Author: Tena Habjanec
• Mentor: Ivana Radojčić Redovniković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2017-01, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Razvoj zelenih otapala važan je zadatak za industriju, pa se stoga se u posljednje vrijeme intenzivno istražuju eutektična otapala zbog njihovih poželjnih karakteristika kao što su niska toksičnost
i biorazgradivost. Eutektična otapala zbog mogućnost prilagodbe njihovih svojstava odabirom početnih sirovina, privukla su pozornost u sintetskoj kemiji i biokatalizi, separacijskim postupcima, ali kao i medij za ekstrakciju prirodnih spojeva. U ovom radu pripremljeni su ekstrakti komine grožđa s osam različitih eutektičnih otapala i s etanolom, zakiseljenim etanolom i vodom. Nakon ekstrakcije provedena je HPLC analiza kako bi se identificirali antocijani koji se nalaze u tako pripremljenim ekstraktima komine grožđa. Eutektična otapala kolin klorid:jabučna kiselina, jabučna kiselina:glukoza, prolin: jabučna kiselina i kolin klorid:prolin:jabučna kiselina pokazuju bolju sposobnost ekstrakcije od za tu namjenu najčešće korištenog organskog otapala, zakiseljenog etanola.

Abstract (english)

Development of the green solvents is a very important task for the industry, so lately eutectic solvents have been intensivly studied because of their desirable characteristics, such as low toxicity and biodegradability. Eutectic solvents has drawn attention in sintetic chemistry and biocatalysis, not only because of their possibility to select the initial raw material with separation procedures but also as a media for extraction of natural compounds. Therefore, in this work, grape pomace extracts are prepared with eight different deep eutectic solvents and ethanol, acidified ethanol and water. Extracts of grape pomace in choline chlorid:malic acid and choline chlorid:proline:malic acid and proline:malic acid have better efficiency of antocyanins extraction what indicates that they are good candidates for further development and usage in the green extraction.

• Keywords: antocijani; ekstrakcija; eutektična otapala; HPLC; komina grožđa
• Keywords (english): anthocyanins; deep eutectic solvents; extraction; grape pomace; HPLC
• Language: croatian
• URN:NBN: urn:nbn:hr:159:410681
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-26 09:34:31