Određivanje prisutnosti alergena lješnjaka u čokoladi i čokoladnim proizvodima

master's thesis

• Title (english): Determination of hazelnut allergens in chocolates and chocolate products
• Author: Iva Radalj
• Mentor: Marina Krpan (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Quality Control); Zagreb
• Defense date and country: 2018-07-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Tijekom ovog istraživanja određeni su potencijalni alergeni lješnjaka u sedam istovrsnih uzoraka čokolada s različitim datumom proizvodnje i sedam različitih uzoraka čokoladnih proizvoda pomoću ELISA (engl. Enzyme-Linked ImmunoSorbent Assay) metode te je ispitan stav potrošača o navodima potencijalne prisutnosti alergena lješnjaka na deklaracijama ispitivanih proizvoda. U svim analiziranim uzorcima detektirana je prisutnost alergena lješnjaka, a određeni udjel nije prelazio >20 mg kg-1. Između analiziranih uzoraka u kojima su određeni alergeni lješnjaka, svi proizvodi su sadržavali navod o potencijalnoj prisutnosti alergena lješnjaka na deklaraciji. Iako samo mali broj ispitanika čita deklaracije na proizvodima, utvrđeno je da navod „Može sadržavati tragove lješnjaka“ 63,5 % ispitanika izdvaja kao najpoznatiji. U ovom istraživanju, u šest analiziranih istovrsnih čokoladnih uzoraka i tri uzorka različitih čokoladnih proizvoda, ELISA metodom analizirani su alergeni lješnjaka.

Abstract (english)

During this research potential hazelnut alergens are defined in seven of the same chocolate samples with different date of production and seven different chocolate samples using ELISA (Enzyme-Linked ImmunoSorbent Assay) method and consumer attitude on allegations of potential presence of hazelnut allergens on declarations of the tested products was examined.. In all analyzed samples hazelnut allergens were detected, but determined content did not exceed amount of >20 mg kg-1. Between analyzed samples in which hazelnut allergens are determinated, all products contained a claim on potential presence of hazelnut allergens on declaration. Although only a small number of respondents read product declarations, it was found that the quote „May contain traces of hazelnuts“ 63,5% of respondents claim as best known. In this research, in six of the same analyzed chocolate samples and three samples of different chocolate products, hazelnut allergnes are analyzed by ELISA method.

• Keywords: alergeni; čokolada; ELISA; lješnjak
• Keywords (english): allergens; hazelnut; ELISA; chocolate
• Language: croatian
• URN:NBN: urn:nbn:hr:159:793462
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-11-26 13:20:24