Ekstrakcija biološki aktivnih spojeva iz ploda kultiviranog komorača (Foeniculum vulgare) primjenom ultrazvuka

undergraduate thesis

• Title (english): Ultrasound assisted extraction of bioactive compounds from the cultivated fennel (Foeniculum vulgare)
• Author: Ivona Marinčić
• Mentor: Zoran Zorić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for drying Technologies and monitoring of biologically active compounds); Zagreb
• Defense date and country: 2021-09-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Cilj ovog rada je bio ispitati utjecaj različitih gnojidbenih tretmana (organsko, mineralno i organsko-mineralno gnojivo) kao i ekstrakcijskog otapala (H2O, 50% etanol, 96% etanol) na udio ukupnih fenola, hidroksicimetnih kiselina te antioksidacijsku aktivnost u ekstraktima odmašćenih sjemenki komorača dobivenih ekstrakcijom potpomognutom ultrazvukom (UAE). Ukupni fenoli, hidroksicimetne kiseline i antioksidacijska aktivnost određene su spektrofotometrijski. Statistička analiza pokazala je da su svi ispitani parametri ekstrakcije imali statistički značajan utjecaj (p≤0,05) na masene udjele ispitivanih spojeva. Najveći prinos ukupnih fenola i hidroksicimetnih kiselina dobiven je kod tretmana s organsko-mineralnim gnojivom, a antioksidacijske aktivnosti kod tretmana mineralnim gnojivom. U ovisnosti upotrebe različitih ekstrakcijskih otapala, najveći prinos ukupnih fenola je dobiven upotrebom vode kao ekstrakcijskog otapala, a hidroksicimetnih kiselina i antioksidacijske aktivnosti upotrebom 96% etanola.

Abstract (english)

The aim of this study was to examine the effect of different fertilization treatments (organic, mineral and organic-mineral fertilizer) and extraction solvents (50% ethanol, 96% ethanol, H2O) on mass fractions of total phenolic compounds, hydroxycinnamic acids and antioxidant activity in extracts obtained from defatted fennel seeds by ultrasound-assisted extraction (UAE). Total phenolic compounds, hydroxycinnamate acids and antioxidant activity were analysed by spectrophotometric methods. Statistical analysis showed that all examined extraction parameters significantly affected (p≤0.05) the mass fractions of the analysed compounds. The highest yield of total phenols and hydroxycinnamic acids was obtained in the treatment with organic-mineral fertilizer, and antioxidant activity in the treatment with mineral fertilizer. Depending on the use of different extraction solvents, the highest yield of total phenols was obtained by using water as the extraction solvent, while hydroxycinnamic acids and antioxidant activity using 96% ethanol.

• Keywords: ultrazvučna ekstrakcija; bioaktivni spojevi; fenolni spojevi; hidroksicimetne kiseline; antioksidacijska aktivnost
• Keywords (english): ultrasound-assisted extraction; bioactive compounds; phenolic compounds; hydroxycinnamic acids; antioxidant activity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:350616
• Study programme: Title: Food Technology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-09-27 10:29:16