Uzgoj kvasaca iz roda Spathaspora i proizvodnja arabitola na hidrolizatu dobivenom kiselinskom predobradom izluženih rezanaca šećerne repe

master's thesis

• Title (english): Spathaspora cultivation and arabitol production on exhausted sugar beet cossettes hydrolysate
• Author: Zora Kelemen
• Mentor: Mario Novak (mentor)
• Committee member: Vlatka Petravić Tominac (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2021-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Kvasci roda Spathaspora imaju sposobnost previranja heksoza i pentoza te se iz tog razloga istražuju za proizvodnju biokemikalija i biogoriva iz lignoceluloznih sirovina. U ovome radu istražen je rast kvasaca Spathaspora passalidarum CBS 10155 i Spathaspora arborarie CBS 11463 na arabinozi pri različitim brzinama okretaja tresilice (50, 100, 150 i 250 o minˉ¹). Također istražena je proizvodnja arabitola na hidrolizatu izluženih repinih rezanaca dobivenom kiselinskom hidrolizom. Uzgojem kvasca Spathaspora passalidarum CBS 10155 na podlozi s arabinozom utvrđeno je da povećanjem broja okretaja iznad 100 o minˉ¹ ne dolazi do značajnog povećanja koncentracije arabitola (2,7873 g Lˉ¹) dok kvascu Spathaspora arborarie CBS 11463 najviše pogoduje brzina od 250 o minˉ¹ za proizvodnju arabitola (4,1491 g Lˉ¹). Uzgojima pri svim brzinama okretaja tresilice utvrđene specifične brzine rasta kvasca Spathaspora arborarie CBS 11463 su manje od kvasca Spathaspora passalidarum CBS 10155. Uzgojem na kiselinskom hidrolizatu kvasac Spathaspora passalidarum CBS 10155 proizvedeno je 8,4810 g Lˉ¹ arabitola (YP/S=0,6038 g gˉ¹, Pr=0,1767 g Lˉ¹ hˉ¹). Na kraju uzgoja kvasca Spathaspora arborarie CBS 11463, koncentracija arabitola iznosila je 2,1759 g Lˉ¹(YP/S=0,5570 g gˉ¹, Pr=0,0453 g Lˉ¹ hˉ¹).

Abstract (english)

Spathaspora yeasts have become of interest for biofuel and biochemicals production from lignocellulose because of their ability to co-ferment hexoses and pentoses. Arabinose fermentation by Spathaspora passalidarum CBS 10155 and Spathaspora arborarie CBS 11463 at different agitation speeds (50, 100, 150 and 250 rpm) was explored in this thesis. In addition, arabitol production from acid hydrolysate of exhausted sugar beet cossettes was investigated. By cultivating Spathaspora passalidarum CBS 10155 in arabinose-rich medium, it was determined that by increasing agitation speed above 100 rpm, there is not a significant increase in arabitol production (2,7873 g Lˉ¹) while the agitation speed of 250 rpm is favoured for arabitol production by Spathaspora arborarie CBS 11463 (4,1491 g Lˉ¹). During all cultivations, specific growth rate of Spathaspora arborarie CBS 11463 was lower than that of Spathaspora passalidarum CBS 10155 at the same agitation speed. When grown on the acid hydrolysate of exhausted sugar beet cossettes, yeast Spathaspora passalidarum CBS 10155 produced 8,4810 g Lˉ¹ of arabitol (YP/S=0,6038 g gˉ¹, Pr=0,1767 g Lˉ¹ hˉ¹) while Spathaspora arborarie CBS 11463 produced 2,1759 g Lˉ¹(YP/S=0,5570 g gˉ¹, Pr=0,0453 g Lˉ¹ hˉ¹).

• Keywords: arabitol; Spathaspora; izluženi repini rezanci; lignoceluloza
• Keywords (english): arabitol; Spathaspora; exhausted sugar beet cossettes; lignocellulose
• Language: croatian
• URN:NBN: urn:nbn:hr:159:707384
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-10-11 10:45:10