Uklanjanje klorofila iz etanolonog ekstrakta lista i cvijeta konoplje

master's thesis

• Title (english): The removal of chlorophyll from ethanol extract of cannabis leaf and flower
• Author: Nikolina Zadro
• Mentor: Marko Obranović (mentor)
• Committee member: Josip Ćurko (predsjednik povjerenstva)
• Committee member: Tibor Janči (član povjerenstva)
• Committee member: Marin Matošić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Oil and Fat Technology); Zagreb
• Defense date and country: 2022-07-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Klorofil je biljni pigment koji se nalazi u zelenim dijelovima biljke i smatra se vizualno i senzorski nepoželjnim u ekstraktu industrijske konoplje. U ovom istraživanju testiran je utjecaj aktivnog ugljena na uklanjanje klorofila. Nakon preliminarnih istraživanja, odabrani su mljeveni pelet aktivnog ugljena i zemlja za bijeljenje. Uklanjanje klorofila rađeno je u etanolnom ekstraktu industrijske konoplje kroz 2 h i mjerenjima svakih 30 min uz različite postotke aktivnog ugljena (1.5 i 10 %). Početne vrijednosti ukupnog klorofila su bile 9.3 mg mLˉ¹, a karotenoida 1.53 mg mLˉ¹. Sadržaj klorofila je smanjen proporcionalno dodanoj količini aktivnog ugljena i vremenu tretmana. Pored klorofila došlo je i do smanjenja udjela karotenoida, kanabidiola (CBD) te ukupnih kanabinoida. Kratki tretmani su se pokazali puno učinkovitiji s obzirom na utjecaj na kanabinoide. Pri dugim tretmanima dolazi do potpunog uklanjanja kanabinoida i karotenoida. Potrebna su daljnja istraživanja radi optimiranja ovog procesa.

Abstract (english)

Chlorophyll is a plant pigment found in its green parts, considered to be visually and sensorially undesirable in the industrial cannabis extract. This research tested the effect of active carbon on chlorophyll removal. After preliminary research, active carbon ground pellet and bleaching earth were chosen. The chlorophyll removal was done in ethanol extract of industrial cannabis in the period of 2 hours with measurements every 30 minutes with different active carbon percentages (1, 5 and 10 %). Initial measures of total chlorophyll were 9.3 mg mLˉ¹, and total carotenoid 1.53 mg mLˉ¹. The chlorophyll content was reduced proportionally to the amount of active carbon added and treatment duration. There was also a reduction in carotenoid portion, cannabidiol (CBD) and total cannabinoids. Short treatments were much more efficient considering the effect on cannabinoids. In other treatments there is a total cannabinoid and carotenoid removal. Further research is needed for optimization.

• Keywords: industrijska konoplja; klorofil; aktivni ugljen; kanabinoidi; CBD
• Keywords (english): industrial cannabis; chlorophyll; activated carbon; cannabinoids; CBD
• Language: croatian
• URN:NBN: urn:nbn:hr:159:700407
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-23 10:24:50