Izolacija i karakterizacija bioaktivnih komponenata iz cvijeta i lista trnine

undergraduate thesis

• Title (english): Isolation and characterization of bioactive components from flower and leaf of blackthorn
• Author: Tonći Kapuralić
• Mentor: Senka Djaković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Organic Chemistry); Zagreb
• Defense date and country: 2016-06-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Trnina (Prunus spinosa L.) je višegodišnja listopadna biljka koji raste gotovo u cijeloj Europi u ekotonu između šumovitih područja i travnjaka, a obiluje bioaktivnim komponentama koje imaju potencijalan protektivan učinak na zdravlje ljudi. U ovom završnom radu provedena je ekstrakcija fenolnih spojeva po Soxhlet-u, a dobiveni ekstrakt pročišćen je i razdvojen pomoću preparativne tankoslojne kromatografije na četiri frakcije koje su karakterizirane primjenom FTIRspektroskopije. Ukupni fenoli,... More flavonoli i hidroksicimetne kiseline određeni su spektrofotometrijski Folin-Ciocalteu metodom, a kvantifikacija etil-acetatnog ekstrakta provedena je tekućinskom kromatografijom visoke djelotvornosti. Na temelju provedenih ispitivanja i dobivenih rezultata može se zaključiti da se u osušenim cvjetovima i listovima trnine nalaze značajne koncentracije fenolnih spojeva koji mogu imati biološki potencijal. Less

Abstract (english)

Blackthorn (Prunus spinosa L.) is a perennial deciduous plant that grows all over Europe in the ecotone between forested areas and grasslands, and is rich in bioactive components that have potential protective effect on human health. In this final work, an extraction of phenolic compounds was conducted by Soxhlet method and resulting extract was purified and separated into four fractions by preparative thin layer chromatography and characterized using FTIR-spectroscopy. Total phenols,... More flavonols and hydroxycinnamic acids were determined using Folin-Ciocalteu spectrofotometric method, while quantification of ethyl acetate extract was carried out using high performance liquid chromatography. Based on the conducted tests and results it can be concluded that dried flowers and leaves of blackthorn contain significant concentrations of phenolic compounds that may have biological potential. Less

• Keywords: osušeni cvijet i list trnine; fenolni spojevi; Soxhlet ekstrakcija; TLC; FTIRspektroskopija; HPLC
• Keywords (english): dried flower and leaf of blackthorn; phenolic compounds; Soxhlet extraction; TLC; FTIRspectroscopy; HPLC
• Language: croatian
• URN:NBN: urn:nbn:hr:159:686874
• Study programme: Title: Nutrition; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 07:40:40