Utjecaj visoknaponskog električnog pražnjenja na prinos sekundarnih biljnih metabolita i proteina iz lista šećerne repe

master's thesis

• Title (english): The influence of high-voltage electric discharge on the yield of secondary plant metabolites and proteins from sugar beet leaves
• Author: Ema Ritoša
• Mentor: Anet Režek Jambrak (mentor)
• Committee member: Sven Karlović (predsjednik povjerenstva)
• Committee member: Anita Slavica (član povjerenstva)
• Committee member: Mojca Čakić Semenčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Management, Foreign Languages for Specific Purposes and Physical Education and Health); (Laboratory for Sustainable Development); Zagreb
• Defense date and country: 2023-07-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Lišće šećerne repe je glavni otpad u proizvodnji šećera te stoga čini potencijalni i održivi izvor proteina i fenolnih spojeva za prehrambenu industriju. Cilj ovog istraživanja je odrediti utjecaj visokonaponskog električnog pražnjenja (eng. High voltage electrical discharge- HVED), na prinos ekstrakcije sekundarnih biljnih metabolita i proteina iz lista šećerne repe te usporediti rezultate s konvencionalnom metodom toplinske ekstrakcije. Ukupni proteini i fenoli u ekstraktima određivani su spektrofotometrijski. HVED potpomognutom ekstrakcijom dobiven je 13,6 % veći prinos proteina u odnosu na toplinsku. Toplinskom ekstrakcijom pri 60 ̊C u trajanju od 3 i 6 minuta dobiveni su 8,5 % veći prinosi ukupnih fenola u odnosu na HVED. Statističkom analizom u programu STATGRAPHICS utvrđeno je da vrijeme tretmana i napon nemaju statistički značajan utjecaj na pH, električnu vodljivost, prinos ukupnih proteina i prinos ukupnih fenola HVED tretiranih uzorka (p > 0,05). Duljina toplinske ekstrakcije ima statistički značajan utjecaj (p < 0,05) na prinos ukupnih proteina i ukupnih fenola.

Abstract (english)

Sugar beet leaves are the main waste of sugar production and therefore constitute a potential and sustainable source of protein and phenolic compounds for the food industry. The aim of this work was to determine the impact of high voltage electrical discharge (HVED) on the extraction yield of secondary plant metabolites and proteins from sugar beet leaves. Obtained results were compared to results of conventional thermal extraction. Total proteins and phenols in the extracts were determined spectrophotometrically. By means of HVED-assisted extraction, 13,6 % higher protein yield was obtained, compared to thermal extraction. For thermal extraction at 60 ̊C for 3 and 6 minutes, an 8,5 % higher yield of total phenols was obtained compared to HVED. Through statistical analysis in the STATGRAPHICS program, it was determined that treatment time and voltage have no statistically significant effect on pH, electrical conductivity, yield of total proteins and yield of total phenols of HVED treated samples (p > 0,05). The length of thermal extraction has a statistically significant effect (p < 0,05) on the yield of total proteins and total phenols.

• Keywords: visokonaponsko električno pražnjenje; list šećerne repe ekstrakcija; proteini; fenoli
• Keywords (english): high voltage electric discharge; sugar beet leaf extraction; proteins; phenols
• Language: croatian
• URN:NBN: urn:nbn:hr:159:391674
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-29 11:22:47