Ekstrakcije bioaktivnih spojeva iz mediteranskog bilja primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku

master's thesis

• Title (english): Accelerated solvent extraction (ASE) of bioactive compounds from Mediterranean herbs
• Author: Lucija Mrmić
• Mentor: Anet Režek Jambrak (mentor)
• Committee member: Jasenka Gajdoš Kljusurić (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Management, Foreign Languages for Specific Purposes and Physical Education and Health); (Laboratory for Sustainable Development); Zagreb
• Defense date and country: 2020-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Kadulja (Salvia officinalis L.), maslina (Olea europaea L.) i origano (Origanum vulgare L.) su mediteranske biljke bogate antioksidansima. Za potrebe ovog diplomskog rada, provedena je ekstrakcija bioaktivnih spojeva iz osušenih uzoraka kadulje, lista masline i origana. Cilj rada je ispitati učinkovitost ubrzane ekstrakcije otapalima pri povišenom tlaku (ASE) pri izolaciji bioaktivnih spojeva navedenog mediteranskog bilja, korištenjem različitih parametara temperature, broja ciklusa te statičkog vremena ekstrakcije. U dobivenim ekstraktima su spektrofotometrijski određeni ukupni fenoli, antioksidacijska aktivnost (DPPH i FRAP metodom) te parametri boje prema CIE L*a*b* sistemu boja. Optimalni uvjeti provođenja ASE ekstrakcije pri kojima je određen najveći udio fenolnih spojeva, antioksidacijska aktivnost te najveće promjene boje u ekstraktima kadulje, lista masline i origana ostvareni su pri parametrima temperature 50 °C, statičkog vremena od 3 min tijekom 3 ciklusa ekstrakcije. Najveći udio fenolnih spojeva izoliran je iz ekstrakta kadulje (34,22 mg GAE gˉ¹), zatim origana (29,53 mg GAE gˉ¹), a najmanji udio iz lista masline (19,14 mg GAE gˉ¹). Statističkom analizom dokazan je značajan utjecaj temperature, statičkog vremena i broja ciklusa na učinkovitost ekstrakcije.

Abstract (english)

Sage (Salvia officinalis L.), olive (Olea europaea L.) and oregano (Origanum vulgare L.) are Mediterranean plants rich in antioxidants. For the purposes of this thesis, the extraction of bioactive compounds from dried samples of sage, olive leaves and oregano were performed. The aim of the study was to examine the efficiency of accelerated solvent extraction (ASE) for the isolation of bioactive compounds of this Mediterranean plants, using different parameters of temperature, number of cycles and static extraction time. In the obtained extracts, total phenols, antioxidant activity (DPPH and FRAP method) and color parameters according to the CIE L*a*b* color system were determined. Optimal conditions for ASE extraction were determined at temperature of 50 °C, static time of 3 min during 3 extraction cycles, in which the highest proportion of phenolic compounds, antioxidant activity and color changes in sage, olive leaf and oregano extracts were observed. The largest proportion of phenolic compounds was isolated from sage extract (34.22 mg GAE gˉ¹), followed by oregano (29.53 mg GAE gˉ¹) and the smallest proportion from olive leaf (19.14 mg GAE gˉ¹). Statistical analysis demonstrated a significant influence of temperature, static time and number of cycles on extraction efficiency.

• Keywords: kadulja; list masline; origano; ASE ekstrakcija; fenolni spojevi; antioksidacijska aktivnost
• Keywords (english): sage; olive leaf; oregano; ASE extraction; phenolic compounds; antioxidant activity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:639454
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-07-14 11:17:24