Izolacija karotenoida iz biootpada kore sušene rajčice uporabom različitih otapala

master's thesis

• Title (english): Isolation of carotenoid from dried tomato peel waste by using different solvents
• Author: Romana Popović
• Mentor: Senka Djaković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Organic Chemistry); Zagreb
• Defense date and country: 2018-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Biootpad rajčice predstavlja bogat izvor nutrijenata i fitokemikalija, između ostalog i karotenoidnih pigmenata, stoga se može iskoristiti za recikliranje u svrhu dobivanja biološki aktivnih spojeva. Ovim radom ispitana je i uspoređena učinkovitost izolacije karotenoida iz biootpada kore sušene rajčice primjenom dviju različitih metoda ekstrakcija, grijanjem u povrat ili refluksiranjem i ekstrakcijom po Soxhlet-u, uz korištenje niza otapala/ smjesa otapala različite polarnosti. Refluksiranjem su dobivene veće mase ekstrakata, kao i uporabom polarnijih otapala što upućuje na to da su temperatura i polarnost otapala važni parametri ekstrakcije. Dobiveni ekstrakti su pročišćeni preparativnom tankoslojnom i kolonskom kromatografijom te karakterizirani IR-spektroskopijom čime je potvrđena prisutnost karotenoida.

Abstract (english)

Tomato waste is considered as a rich nutrient source containing phytochemicals, among which are carotenoids pigments, therefore it can be recycled for the uses of biologically active compounds. This thesis carried out and compared carotenoid isolation efficiency from dried tomato peel waste by applying two different extraction methods, classical extraction or refluxing and Soxhlet extraction, using a series of solvents of different polarity. Larger amounts of extracts were obtained by refluxing, as well as with more polar solvents indicating that temperature and solvent polarity are important extraction parameters. The extracts were purified by preparative thin layer and column chromatography, then characterized by IR-spectroscopy which confirmed the presence of carotenoids.

• Keywords: biootpad; rajčica; izolacija; karotenoidi; IR-spektroskopija
• Keywords (english): biowaste; tomato; isolation; carotenoids; IR-spectroscopy
• Language: croatian
• URN:NBN: urn:nbn:hr:159:509386
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-12-03 11:04:29