Odabir "zelenih" otapala u ekstrakcijama bioaktivnih komponenata i eteričnih ulja putem digitalnog predviđanja

master's thesis

• Title (english): Selection of “green” solvents in extractions of bioactive components and essential oils by digital prediction
• Author: Ana Kovač
• Mentor: Anet Režek Jambrak (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Food Processes Engineering); Zagreb
• Defense date and country: 2019-10-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Cilj ovog istraživanja bio je provesti ekstrakcije bioaktivnih komponenata iz kadulje (Salvia officinalis L.) i origana (Origanum vulgare L.) klasičnom ekstrakcijom i ekstrakcijom ultrazvukom visoke snage. Naglasak je bio utvrditi te odabrati zelena otapala za provođenje ekstrakcija, te predvidjeti topivost bioaktivnih komponenata u otapalima što je utvrđeno putem Conductor-like Screening Model for Real Solvents (COSMO-RS) i Hansen softverskim paketima. Nadalje, cilj je bio eksperimentalno utvrditi predviđene rezultate topljivosti. Tijekom istraživanja uzorci kadulje (Salvia officinalis L.) i origana (Origanum vulgare L.) tretirani su ultrazvukom visoke snage pri amplitudi 75 i 100 %. Ekstrakcija tretiranih i netretiranih uzoraka (1 g uzorka u 50 mL otapala) provodila se 3 i 9 min s vodom, 25 %-tnim i 50 %-tnim etanolom te etil-acetatom, dimetil karbonatom, limonenom i α- pinenom. Najveći prinos ukupnih fenola dobiven je kod uzorka origana tretiranog 3 min pri amplitudi od 100 % u otopini 25 %-tnog etanola. Korištenjem metode ultrazvuka dokazana je viša antioksidacijska aktivnost uzoraka u usporedbi s konvencionalnom metodom.

Abstract (english)

The aim of this study was to conduct extractions of bioactive components from sage (Salvia officinalis L.) and oregano (Origanum vulgare L.) using classical extraction and extraction by means of high power ultrasound. The goal was to identify and select the green solvents that can be used for further extractions, and to predict the solubility of the bioactive components in solvents as determined through "Conductor-like Screening Model for Real Solvents (COSMO-RS)" and "Hansen" softwares. Furthermor, the aim was to experimentally determine/evaluate the predicted solubility results. Samples of sage (Salvia officinalis L). and oregano (Origanum vulgare L.) were treated by means of high power ultrasound using amplitude rate of 75 % and 100 %. Extraction of treated and untreated samples (1 g of sample in 50 mL of solvent) were treated for 3 and 9 min using solvents: water, 25 % and 50 % ethanol and ethyl acetate, dimethyl carbonate, limonene and α-pinene. Largest yield of total phenolics was obtained with an oregano sample that was treated for 3 min, using amplitude of 100 % in a solution of 25 % ethanol. Utilization of ultrasound method showed higher antioxidant activity of the samples, as compared to the samples that were subjected to the conventional method.

• Keywords: kadulja; origano; COSMO-RS; Hansen-ovi parametri topljivosti; ukupni fenoli; antioksidacijska aktivnost
• Keywords (english): sage; oregano; COSMO-RS; Hansen solubility parameters; total phenolic compounds; antioxidant activity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:565015
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-12-11 13:18:58